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Original Research 
RMJ. 2022; 47(2): 315-318


Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard

Mohammad Sajjad Khattak, Zubaida Khanum, Naseeb ur Rehman Shah, Rafi Ullah Khattak, Abdul Ghafoor, Asif Mehmood.

Abstract
Objective: To see the frequency and to compare the sensitivity, specificity and negative predictive values of Acid Fast Bacilli by Ziehl Neelsen and Auramine Flourescent microscopy in pulmonary tuberculosis.
Methodology: This descriptive cross sectional study was done at Pathology Department, Khalifa Gul Nawaz Teaching Hospital, Bannu, KPK, Pakistan from January 2012 to December 2013. A total of 1000 sputum specimen were selected by non-probability random sampling technique. Gene Xpert was ready for analysis of the sample. Result after two hours was plotted as 1- Positive/negative for Acid Fast Bacilli. 2- Rifampicin sensitive/resistant. All specimens were subjected to both Ziehl Neelsen staining as well as Flourescent staining with Auramine to see their comparative positive yield. Chi Square test was used to compare yield of Ziehl Neelsen stain and Auramine fluorescent stain.
Results: All Gene Xpert positive cases were positive on Flourescent Microscopy as well as Zieh Neelsen Technique and all F.M. positive cases were positive by Z.N. technique (p<0.05).
Conclusion: Flourescent Microscopy of Mycobacterium TB has better yield as compared to conventional Ziehl Neelsen microscopy keeping Gene Xpert as gold standard for mycobacterial diagnosis.

Key words: Sputum AFB, pulmonary tuberculosis, Gene Xpert, fluorescent microscopy, Zeil Nelson stain.


 
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How to Cite this Article
Pubmed Style

Khattak MS, Khanum Z, Shah NuR, Khattak RU, Ghafoor A, Mehmood A. Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. RMJ. 2022; 47(2): 315-318.

Web Style

Khattak MS, Khanum Z, Shah NuR, Khattak RU, Ghafoor A, Mehmood A. Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. https://www.rmj.org.pk/?mno=91881 [Access: December 05, 2023].

AMA (American Medical Association) Style

Khattak MS, Khanum Z, Shah NuR, Khattak RU, Ghafoor A, Mehmood A. Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. RMJ. 2022; 47(2): 315-318.


Vancouver/ICMJE Style

Khattak MS, Khanum Z, Shah NuR, Khattak RU, Ghafoor A, Mehmood A. Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. RMJ. (2022), [cited December 05, 2023]; 47(2): 315-318.


Harvard Style

Khattak, M. S., Khanum, . Z., Shah, . N. u. R., Khattak, . R. U., Ghafoor, . A. & Mehmood, . A. (2022) Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. RMJ, 47 (2), 315-318.


Turabian Style

Khattak, Mohammad Sajjad, Zubaida Khanum, Naseeb ur Rehman Shah, Rafi Ullah Khattak, Abdul Ghafoor, and Asif Mehmood. 2022. Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. Rawal Medical Journal, 47 (2), 315-318.


Chicago Style

Khattak, Mohammad Sajjad, Zubaida Khanum, Naseeb ur Rehman Shah, Rafi Ullah Khattak, Abdul Ghafoor, and Asif Mehmood. "Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard." Rawal Medical Journal 47 (2022), 315-318.


MLA (The Modern Language Association) Style

Khattak, Mohammad Sajjad, Zubaida Khanum, Naseeb ur Rehman Shah, Rafi Ullah Khattak, Abdul Ghafoor, and Asif Mehmood. "Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard." Rawal Medical Journal 47.2 (2022), 315-318. Print.


APA (American Psychological Association) Style

Khattak, M. S., Khanum, . Z., Shah, . N. u. R., Khattak, . R. U., Ghafoor, . A. & Mehmood, . A. (2022) Frequency and comparison of validity parameters of sputum Acid Fast Bacilli on Ziehl Neelsen and auramine fluorescent microscopy in pulmonary tuberculosis keeping Gene Xpert as gold standard. Rawal Medical Journal, 47 (2), 315-318.


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