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2025, Vol: 50, Issue: 2
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Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH)

Danny Irawan, Gondo Mastutik, Muhammad Miftahussurur, Akbar Reza Muhammad, Lysa Veterini, Soetjipto Soetjipto.


Abstract
Objective: To evaluate the effect of different diets and induction methods on lipid metabolism and liver histopathology in C57BL/6J mice.
Methodology: Twenty-four C57BL/6J mice (8-12 weeks old) were divided into four groups: normal diet (K), Western diet with CCl4 (0.2 µl/kg BW, IP, twice weekly) (P1), Western diet alone (P2), and CDAHFD (P3). The diets were administered for 8 weeks. After 8 weeks, liver and blood samples were collected and analyzed using one-way ANOVA and the Kruskal-Walli’s test.
Results: The WD + CCl4 group had the lowest lipid profile (total cholesterol: 49.80±10.52, HDL: 21.80±8.10, triglycerides: 87.00±47.13) but elevated ALT and AST levels, indicating severe liver inflammation. In contrast, the CDAHFD group had the highest lipid levels (cholesterol: 152.6±20.89, HDL: 96.80±11.56, triglycerides: 216.4±56.49) without signs of inflammation. More than 50% of the WD + CCl4 group tested positive for NASH and had the highest NAS scores (p<0.05).
Conclusion: The WD + CCl4 combination effectively induces NASH, while CDAHFD was better suited for modeling Non-Alcoholic Fatty Liver Disease.

Key words: Non-alcoholic steatohepatitis, Western diet, choline-deficient diet, high-fat diet, non-alcoholic fatty liver disease.


 
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How to Cite this Article
Pubmed Style

Irawan D, Mastutik G, Miftahussurur M, Muhammad AR, Veterini L, Soetjipto S. Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). RMJ. 2025; 50(2): 488-491. doi:10.5455/rmj.20241220065347

Web Style

Irawan D, Mastutik G, Miftahussurur M, Muhammad AR, Veterini L, Soetjipto S. Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). https://www.rmj.org.pk/?mno=233860 [Access: May 12, 2025]. doi:10.5455/rmj.20241220065347

AMA (American Medical Association) Style

Irawan D, Mastutik G, Miftahussurur M, Muhammad AR, Veterini L, Soetjipto S. Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). RMJ. 2025; 50(2): 488-491. doi:10.5455/rmj.20241220065347


Vancouver/ICMJE Style

Irawan D, Mastutik G, Miftahussurur M, Muhammad AR, Veterini L, Soetjipto S. Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). RMJ. (2025), [cited May 12, 2025]; 50(2): 488-491. doi:10.5455/rmj.20241220065347


Harvard Style

Irawan, D., Mastutik, . G., Miftahussurur, . M., Muhammad, . A. R., Veterini, . L. & Soetjipto, . S. (2025) Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). RMJ, 50 (2), 488-491. doi:10.5455/rmj.20241220065347


Turabian Style

Irawan, Danny, Gondo Mastutik, Muhammad Miftahussurur, Akbar Reza Muhammad, Lysa Veterini, and Soetjipto Soetjipto. 2025. Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). Rawal Medical Journal, 50 (2), 488-491. doi:10.5455/rmj.20241220065347


Chicago Style

Irawan, Danny, Gondo Mastutik, Muhammad Miftahussurur, Akbar Reza Muhammad, Lysa Veterini, and Soetjipto Soetjipto. "Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH)." Rawal Medical Journal 50 (2025), 488-491. doi:10.5455/rmj.20241220065347


MLA (The Modern Language Association) Style

Irawan, Danny, Gondo Mastutik, Muhammad Miftahussurur, Akbar Reza Muhammad, Lysa Veterini, and Soetjipto Soetjipto. "Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH)." Rawal Medical Journal 50.2 (2025), 488-491. Print. doi:10.5455/rmj.20241220065347


APA (American Psychological Association) Style

Irawan, D., Mastutik, . G., Miftahussurur, . M., Muhammad, . A. R., Veterini, . L. & Soetjipto, . S. (2025) Comparative effects of diets and induction methods on lipid metabolism and liver histopathology in a mouse model of non-alcoholic steatohepatitis (NASH). Rawal Medical Journal, 50 (2), 488-491. doi:10.5455/rmj.20241220065347


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