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Original Research 
RMJ. 2017; 42(3): 396-399


ILED fluorescent microscope in laboratory diagnosis of tuberculosis

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel.


Abstract
Objective: To assess new fluorescent technique in comparison with conventional ZN technique to improve detection of mycobacterium tuberculosis (TB) in Sudan.
Methodology: This case control study was conducted at Khartoum state Hospitals during December 2010. Three sputum samples were collected on 2 consecutive days from each one of 80 patient and was stained by ZN stain and fluorescent stain.
Results: From 80 patients suspected of tuberculosis, proportion of positive smears that detected were 17% and 33% for the ZN and auramine – O staining methods. The mean time of ZN technique in reading the result was 101 sec compared with fluorescent technique in reading of the result was 33.1 sec.
Conclusion: Fluorescent stain is more sensitive by 16% than ZN stain for diagnosis of TB. Fluorescent stain is three times rapid in detection more than ZN stain. Using this method to diagnose TB will be easy and less time consuming and will give accurate result.

Key words: ILED fluorescent microscope, laboratory analysis, tuberculosis.


 
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How to Cite this Article
Pubmed Style

Abbas AOI, Elrayah EE, Gameel FMH. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ. 2017; 42(3): 396-399.

Web Style

Abbas AOI, Elrayah EE, Gameel FMH. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. https://www.rmj.org.pk/?mno=257134 [Access: June 27, 2026].

AMA (American Medical Association) Style

Abbas AOI, Elrayah EE, Gameel FMH. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ. 2017; 42(3): 396-399.


Vancouver/ICMJE Style

Abbas AOI, Elrayah EE, Gameel FMH. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ. (2017), [cited June 27, 2026]; 42(3): 396-399.


Harvard Style

Abbas, A. O. I., Elrayah, . E. E. & Gameel, . F. M. H. (2017) ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ, 42 (3), 396-399.


Turabian Style

Abbas, Amin Omer Ibrahim, Entisar Elhaj Elrayah, and Fathelrahman Mahd Hassan Gameel. 2017. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. Rawal Medical Journal, 42 (3), 396-399.


Chicago Style

Abbas, Amin Omer Ibrahim, Entisar Elhaj Elrayah, and Fathelrahman Mahd Hassan Gameel. "ILED fluorescent microscope in laboratory diagnosis of tuberculosis." Rawal Medical Journal 42 (2017), 396-399.


MLA (The Modern Language Association) Style

Abbas, Amin Omer Ibrahim, Entisar Elhaj Elrayah, and Fathelrahman Mahd Hassan Gameel. "ILED fluorescent microscope in laboratory diagnosis of tuberculosis." Rawal Medical Journal 42.3 (2017), 396-399. Print.


APA (American Psychological Association) Style

Abbas, A. O. I., Elrayah, . E. E. & Gameel, . F. M. H. (2017) ILED fluorescent microscope in laboratory diagnosis of tuberculosis. Rawal Medical Journal, 42 (3), 396-399.


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