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Original Research 
RMJ. 2024; 49(3): 661-664


In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line

Hafiz Muhammad Haseeb Khaliq, Owais Aziz, Monika Wadhwa, Shahla Imran.


Abstract
Objective: To investigate the pro-apoptotic potential of capsaicin on Human Leukemia HL-60 cell line as a potent natural chemotherapeutic agent.
Methodology: This study was conducted at Forman Christian College, Lahore from January to April-2024. It included culturing HL-60 cell line and its treatment with serial concentrations of capsaicin for calculation of subsequent IC50 values via MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay, expression analysis of gene, comparative analysis of relative gene fold (Intrinsic Biomarkers Caspase-3, Caspase-9) as apoptosis mediator markers followed by RT-qPCR. Cellular apoptotic morphology was confirmed via Hoechst 333258 staining.
Results: For HL-60 cell line, the IC50 of capsaicin (16.7uM) showed high gene expression of pro-apoptotic biomarkers (Caspase-3 and Caspase-9). Hoechst 333258 staining showed prominent apoptotic bodies leading to nuclear fragmentations.
Conclusion: Capsaicin had robust pro-apoptotic potential leading to diagnostic efficacy against leukemia HL-60 cell line. Further studies would help in identifying key mechanisms by which capsaicin exhibits anti-apoptotic potential against HL-60 cell lines.

Key words: Capsaicin, pro-apoptotic effects, anti-cancer mechanisms, HL-60, natural chemotherapeutics.


 
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How to Cite this Article
Pubmed Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ. 2024; 49(3): 661-664.

Web Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. https://www.rmj.org.pk/?mno=201867 [Access: September 12, 2024].

AMA (American Medical Association) Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ. 2024; 49(3): 661-664.


Vancouver/ICMJE Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ. (2024), [cited September 12, 2024]; 49(3): 661-664.


Harvard Style

Khaliq, H. M. H., Aziz, . O., Wadhwa, . M. & Imran, . S. (2024) In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ, 49 (3), 661-664.


Turabian Style

Khaliq, Hafiz Muhammad Haseeb, Owais Aziz, Monika Wadhwa, and Shahla Imran. 2024. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. Rawal Medical Journal, 49 (3), 661-664.


Chicago Style

Khaliq, Hafiz Muhammad Haseeb, Owais Aziz, Monika Wadhwa, and Shahla Imran. "In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line." Rawal Medical Journal 49 (2024), 661-664.


MLA (The Modern Language Association) Style

Khaliq, Hafiz Muhammad Haseeb, Owais Aziz, Monika Wadhwa, and Shahla Imran. "In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line." Rawal Medical Journal 49.3 (2024), 661-664. Print.


APA (American Psychological Association) Style

Khaliq, H. M. H., Aziz, . O., Wadhwa, . M. & Imran, . S. (2024) In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. Rawal Medical Journal, 49 (3), 661-664.


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